Bioluminescent Assay for Determination of Picomole Amounts of AMP, ADP, and ATP in Adipose and Muscle Tissue Biopsies

M. Feraudi; J. Merkt; H. Weicker

doi:10.1055/s-2008-1034592

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 1981; 02(2): 106-109
DOI: 10.1055/s-2008-1034592

Bioluminescent Assay for Determination of Picomole Amounts of AMP, ADP, and ATP in Adipose and Muscle Tissue Biopsies

M. Feraudi, J. Merkt, H. Weicker

Department of Internal Medicine, Institute of Pathophysiology and Sports Medicine, University of Heidelberg, FRG

The authors wish to thank Miss Carmen Gärtner for skillful technical assistance.

Abstract

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Abstract

A method is described for quantitative determinations of AMP, ADP, and ATP in tissue samples weighing a few milligrams by utilizing constant bioluminescent light intensity after reagent mixing. The smallest measurable quantity of adenosine nucleotide amounts to 0.2 pmol (coefficient of variation = 0.05) in the bioluminescent assay.

Key words

AMP - ADP - ATP - bioluminescent assay - firefly luciferase - adipose tissue biopsy - muscle biopsy

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